Qtl mapping in wheat pdf

Frontiers qtl mapping of grain zn and fe concentrations in. After removing snps that were monomorphic, with 10% missing rate, and with distorted segregation, 6185 polymorphic markers, comprising 2005 for the a genome, 2170 for the b genome and 2010 for the d genome, were used to construct linkage maps of 1767. These traits are usually controlled by quantitative trait loci. Rflp mapping of qtl for fusarium head blight resistance in wheat. The 5 and 10 tp results correspond to the systematic r 1 and r 5 scenarios see table 1. Identification of a major qtl and associated molecular. Molecular markers provide a rapid approach to breeding for desired traits.

Oct 11, 2018 highdensity genetic linkage maps are essential for precise mapping quantitative trait loci qtl in wheat triticum aestivum l. Highdensity genetic linkage maps are essential for precise mapping quantitative trait loci qtl in wheat triticum aestivum l. For linkage map construction and qtl mapping of the ag population, which is a 3way. Shyamal krishna talukder 0 2 md ali babar 1 kolluru vijayalakshmi 0 jesse poland 4 pagadala venkata vara prasad 0 robert bowden 3 allan fritz 0 0 department of agronomy, kansas state university, manhattan, ks 66506, usa 1 department of agronomy, university of florida, gainesville, florida. Sep 11, 2007 kumar n, kulwal pl, balyan hs, gupta pk. Pdf low phytate qtl mapping in winter wheat triticum. Article pdf available in theoretical and applied genetics 14 january 2018 with 249 reads how we measure reads. Qtl 9 environments interaction introduction wheattriticumaestivuml. It utilizes multiple marker intervals simultaneously to fit multiple putative qtl directly in the model for mapping qtl. Functional qtl mapping and genomic prediction of canopy. Rflp mapping of qtl for fusarium head blight resistance in.

Pdf qtl mapping of flag leafrelated traits in wheat. Highdensity genetic linkage map construction and qtl mapping. Highdensity genetic linkage map construction and qtl. Conditional qtl mapping for waterlogging tolerance in two. Qtl mapping was performed using inclusive composite interval mapping of additive icimadd and epistatic qtl icimepi functions in qtl icimapping version 3. Two qtl were associated with type i resistance 6d and 7b, and two were associated with type ii resistance 1b and. This study was conducted to investigate the genetic basis of. Qtl mapping of kernel traits and validation of a major qtl for. Markers associated with a qtl for grain yield in wheat under drought. An ssreststs marker map was con structed and a grain yield qtl on the proximal region of chromosome 4al was found to have a significant impact on.

A multiparental cross population for mapping qtl for. Data for grain area size gas, grain width gwid, factor form density ffd, grain lengthwidth ratio glwr, thousand grain weight tgw, grain perimeter length gpl and grain. Resistance to septoria tritici blotch stb is an economically important trait in many wheat breeding programs across the world. Genetic mapping reveals largeeffect qtl for anther extrusion in cimmyt spring wheat. Ai single and jr multiple qtl approach based on multitrait hklod, maximum mlod, and average slod profiles. A quantitative trait locus qtl is a locus section of dna that correlates with variation of a quantitative trait in the phenotype of a population of organisms. Qtl mapping of kernel traits and validation of a major qtl for kernel lengthwidth ratio using snp and bulked segregant analysis in wheat. Single nucleotide polymorphism snp markers are commonly used in quantitative trait loci. Wild emmer wheat, triticum dicoccoides, is the progenitor of modern tetraploid. The final audpc values were analyzed to obtain the additive qtls and epistatic qtls for wheat fhb resistance, using a 1. Qtl mapping for seedling morphology under drought stress in wheat cross synthetic w7984opata volume 16 issue 4 maria khalid, alvina gul, rabia amir, mohsin ali, fakiha afzal, umar masood quraishi, zubair ahmed, awais rasheed. Linkage analysis and qtl mapping are two closely related aspects of genetic studies but have hitherto been handled by separate software packages tables s2 and s3.

Mapping quantitative trait loci controlling agronomic. Whole genome mapping in a wheat doubled haploid population using ssrs and traps and the identi. Several quantitative trait loci qtl for stb resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genesqtl and determine the underlying resistance mechanism. Diab2 1department of plant physiology, faculty of agriculture, cairo university, giza, egypt 2agricultural genetic engineering research institute, agricultural research center, giza, egypt faculty of biotechnology, october university of modern sciences and art msa, egypt. Qtl mapping for seedling morphology under drought stress. Utilization of molecular markers for pgrfa characterization and prebreeding for climate changes aug. Qtl mapping for some grain traits in bread wheat triticum.

Qtl mapping for early vigor related traits in an elite wheat breeding parent chuanmai 42 derived from synthetic hexaploid wheat jun li1, hongshen wan1, huiting wei2, qin wang1, yonghong zhou3, and wuyun yang1, 1key laboratory of wheat biology and genetic improvement on southwestern china ministry of agriculture. In the present study, quantitative trait loci qtl regulating grain traits in wheat were identified. Figures were drawn using icimadd and icimepi methods. Qtl mapping of kernel traits and validation of a major qtl. Advanced backcross qtl analysis of a hard winter wheat.

Mapping qtl for the traits associated with heat tolerance in wheat triticum aestivum l. Qtl mapping for yieldrelated traits in wheat based on four. Pdf conditional qtl mapping of protein content in wheat with. Till many years qtl mapping was considered as a powerful tool for genetic. Molecular markers, mapping populations and statistical methods ashok kumar meena1, m. Domestication quantitative trait loci in triticum dicoccoides. The spring wheatderived qtl fhb1 was successfully introgressed into triticale and resulted. Quantitative trait loci qtl analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of qtl analysis. Qtl a gene or chromosomal region that affects a quantitative trait. Mapping qtl for the traits associated with heat tolerance in. Qtl information, additional mapping populations are needed to obtain a more complete picture of the diverent genetic factors avecting pasta quality traits in the modern durum germplasm. However, df is depleted in white wheat flour which is more widely consumed than wholegrain.

For example, to use mapqtl for qtl mapping, one needs first to use joinmap to build linkage maps. In the 1990s, the first wheat genetic map was successfully. Frequency distributions and composite interval mapping for qtl analysis in steptoe x morex barley mapping population. Resistance to septoria tritici blotch stb is an economically important trait in many wheatbreeding programs across the world. Identification of a major qtl and associated molecular marker. Mapping qtl for agronomic traits on wheat chromosome 3a and a. Qtl mapping for some grain traits in bread wheat triticum aestivum l. Frontiers qtl mapping of grain zn and fe concentrations. Diab2 1department of plant physiology, faculty of agriculture, cairo university, giza, egypt 2agricultural genetic engineering research institute, agricultural research center, giza, egypt. It also determines interaction of significant qtls and their. Triticeae mapping initiative itmi population somers. The map constructed will facilitate qtl and fine mapping of quantitative traits, mapbased cloning, comparative mapping, and in markerassisted wheat breeding endeavors. Qtl mapping for yieldrelated traits in wheat based on. Whole genome mapping in a wheat doubled haploid population.

Four qtl were detected for fhb resistance under field conditions on chromosomes 1b, 2d, 6d, and 7b. To date, qtls for grain shape and size have been detected on almost all 21 wheat chromosomes 3,4,912,20,2225,27,38,39,5053. It must be polymorphic have allelic variation to have an effect in a population and it must be linked to a polymorphic marker allele to be detected. Article pdf available in physiology and molecular biology of plants 241 june 2018 with 398 reads how we measure reads. Mapping quantitative trait loci qtl in progeny derived from cross aa x.

Recombinant inbred lines with these two qtl had a median severity of 20. Author links open overlay panel takele weldu gebrewahid a b 1 peipei zhang a 1 yue zhou c 1 xiaocui yan a xianchun xia d zhonghu he d e daqun liu a zaifeng li a. Qtls for grain shape and size tgw has been subjected to qtl analysis in many studies but very limited information is available for qtl mapping of gl, gw and gt in wheat. Over the last 10 years, we have developed integrated software called qtl icimapping. Qtl analysis for some quantitative traits in bread wheat. Shyamal krishna talukder1,3, md ali babar2, kolluru vijayalakshmi3, jesse poland4, pagadala venkata vara prasad3, robert bowden5 and allan fritz3 abstract. To wll this gap in our understanding of the genetic factors underlying important quality parameters we developed a genetic map between adapted durum wheat. Dietary fibre df has multiple health benefits and wheat grains are major sources of df for human health. Qtl mapping of flag leafrelated traits in wheat triticum aestivum l.

Heading date qtl in winter wheat triticum aestivum l. Agronomy free fulltext qtl mapping and transcriptome. Total of 36 qtls were identified on 18 chromosomes in itmi population, and they explained 0. Qtl mapping for quality traits using a highdensity. Analysis of genetic control and qtl mapping of essential wheat grain quality traits in a.

The linkage map used for analysis was based on the marker order and relative distances in the international. About 1015 million ha of the worlds wheat growing areas are affected by waterlogging each year sayre et al. A tool for improvement in crop plants shaukeen khan department of plant breeding and genetics, mpuat, udaipur, rajasthan 301, india available online at. Bmc genetics mapping qtl for the traits associated with heat tolerance in wheat triticum aestivum l. Qtl mapping for yield and yield contributing traits in two mapping populations of bread wheat. Linkage loci that are close enough together on the same chromosome to deviate from independent assortment are said to display genetic linkage 4. Qtl mapping of adult plant resistance to stripe rust and. Genomic resources and geneqtl discovery in cereals. Qtl mapping of adult plant resistance to stripe rust and leaf rust in a fuyu 3zhengzhou 5389 wheat population. In several mapping populations derived from chinese wheat lines, a significant type 2 fhb. Several quantitative trait loci qtl for stb resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes qtl and determine the underlying resistance mechanism. Nov 27, 2017 the map constructed will facilitate qtl and fine mapping of quantitative traits, mapbased cloning, comparative mapping, and in markerassisted wheat breeding endeavors. In this study, a highdensity genetic linkage map consisted of 6312 snp and ssr markers was developed to identify qtl controlling kernel size and weight, based on a recombinant inbred line ril population derived from the cross of shixin828 and kenong2007.

Mapping qtl for the traits associated with heat tolerance. Qtl mapping and successful introgression of the spring wheat. Pdf qtl mapping for some grain traits in bread wheat. Mapping agronomic and quality traits in elite durum wheat. Molecular markers xbarc1 and aflp agctcg347 on chromosome 6a, xbarc121 and xbarc49 on 7a, gwm18 and bin1 on 1b, bin178 and bin81 on 2b and bin747 and bin1546 on 1d were associated with these qtl. Research article open access mapping qtl for the traits associated with heat tolerance in wheat triticum aestivum l. Cim results in terms of functional haplotypes, we identified on average 2. Qtl mapping of adult plant resistance to stripe rust and leaf. Locations of markers goals identify the or at least one genomic region, called quantitative trait locus qtl, that contributes to variation in the trait. This is often an early step in identifying and sequencing the actual genes that cause the trait variation. Radiation hybrid qtl mapping of tdes2 involved in the. The snps in 2a are most likely related to the major qtl, and there may be minor. We constructed a highdensity genetic map containing 10,739 loci 5,399 unique loci using a set of 184 recombinant inbred lines rils derived from a cross of tainong 18. Qtls are mapped by identifying which molecular markers such as snps or aflps correlate with an observed trait.

The barley genetics newsletter is published electronically at. The authors are grateful for higher education commission. Qtl mapping of adult plant resistance to ug99 stem rust in. Original paper radiation hybrid qtl mapping of tdes2 involved in the. Electronic supplementary material the online version of. Functional qtl mapping analysis for five, 10, and 26 time points tps in 2017. These traits are usually controlled by quantitative trait loci qtls and are highly in. A key test weight qtl was detected on 3b and prominent grain weight qtls were identified on 3d and 4a. Yield and yieldrelated traits are important factors in wheat breeding. Qtl detection for kernel size and weight in bread wheat. Grain traits are important agronomic attributes with the market value as well as milling yield of bread wheat. The best rflp marker in the 3bs region explained 15. Analysis for grain protein content gpc suggested that the major part of genetic variation for this trait is due to environmental interactions. In this study, four ril populations derived from the cross of one common parent yanzhan 1 a chinese domesticated.

Plasticity of the flowering pathway in wheat is key to adaptation as it allows for regulation of developmental phases in different environments and accounts for the broad geographical range of wheat cultivation. May 27, 20 waterlogging is a widespread limiting factor for wheat production throughout the world specially irrigated and high rainfall environments. The identified qtl can be used for marker assisted selection in breeding wheat for improved heat tolerance in ventnor or karl 92 genetic background. Keywords linkage mapping recombinant inbred lines stem rust ug99 genotyping by sequencing resistance breeding key message this study reports the detection of qtl associated with resistance to the ug99 race group of stem rust of wheat, in us spring wheat breeding germplasm. Analysis of genetic control and qtl mapping of essential wheat. Research article open access mapping qtl for the traits. Eight environmentally stable qtl for grain yieldrelated traits were detected by four ril populations, and two of them were validated by a natural wheat population containing 580 diverse varieties or lines. Qtl mapping studies have been reported in most crop plants for diverse traits including yield, quality, disease and insect resistance, abiotic stress tolerance, and environmental adaptation.

Genetic studies of qtl mapping quantitative trait loci mapping based on finescale linkage maps have greatly increased our understanding of the inheritance of quantitative traits in the last 20 years. Qtl mapping for quality traits using a highdensity genetic. Mapping qtl for agronomic traits on wheat chromosome 3a. Genetic mapping reveals largeeffect qtl for anther. Pdf qtl mapping for some grain traits in bread wheat triticum. The mapping of quantitative traits in wheat is complicated due to polyploidy and large genome size, estimated to be around 16,000 mbp1c arumuganathan and earle, 1991, about 85 to 96% of which represents mapping qtl for agronomic traits on wheat chromosome 3a and a comparison of recombinant. Here, a brief overview of the principle of qtl mapping, salient requirements for qtl mapping, common statistical tools and techniques employed in qtl analysis. Genomewide association analyses for yield and yield. Pdf grain protein content in wheat triticum aestivum l. Qtl mapping for seedling morphology under drought stress in. To use them, it is necessary to determine the linkage between quantitative trait loci qtl and such markers. Multiple interval mapping mim it is also a modification of simple interval mapping.

We have identified the chinese wheat cultivar yumai 34 as having unusually high contents of ax in both water. Mapping quantitative trait loci controlling agronomic traits. Mapping qtl for agronomic traits in breeding populations. A linkage map of 55 simple sequence repeat markers for 8 wheat chromosomes was used for qtl analysis by composite interval mapping. Information identified by qtl mapping is important for fine gene mapping, mapbased cloning, and efficient use of gene information in molecular breeding.